By Alton Meister
Advances in Enzymology and similar parts of Molecular Biology is a seminal sequence within the box of biochemistry, supplying researchers entry to authoritative experiences of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, delivering an unmatched view of the old improvement of enzymology. The sequence bargains researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic strategy, and their program in either the laboratory and undefined. each one quantity within the sequence positive aspects contributions through major pioneers and investigators within the box from world wide. All articles are rigorously edited to make sure thoroughness, caliber, and clarity.
With its wide selection of issues and lengthy historic pedigree, Advances in Enzymology and similar components of Molecular Biology can be utilized not just by means of scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally by means of any scientist drawn to the invention of an enzyme, its houses, and its functions.
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Extra resources for Advances in Enzymology and Related Areas of Molecular Biology, Volume 23
Kendrew, J. , G . Bodo, H. M. Dintzis, R. 0. I'arrish, H. W. Wyckoff, and 1). C. Phillips, Nature, 181, 662 (1958). 5. , H. M. Dintzis, J. C. Kendrew, and H. W. Wyckoff, Proc. Roy. , A25S, 70 (1959). 6 . Kendrew, J. , R. E. Dickerson, B. E. Strandberg, R. G. Hart, D. R. Davies, D. C. Phillips, and V. C. Shore, Nature, 185, 422 (1960). 7. , Spectra of Diatomic Molecules, Van Nostrand, New York, 1950. 8. , and T. Shimanouchi, Infrared Absorption and Raman Effect, Kyoritsu Shuppan, Tokyo, 1958. 9.
In summarizing the above results, it appears that DPd is necessary not only for the appearance of enzyme action but also for stabilization of the conformation of the dehydrogenase molecule. This idea is supported also by evidence (100) showing that the reduced acetylpyridine analogue of DPN is more firmly bound to bovine heart LDH than the native coenzyme. Thus the former is less susceptible to proteinase digestion during heat treatment than the latter. V. Eflect of Coenzymes and Substrates on Denaturation of Dehydrogenases Kunitz and McDonald (56) showed that, in the absence of its substrate, hexokinase crystallized from baker’s yeast can be rapidly digested by trypsin and proteolytic enzymes present in the yeast cells, although in the presence of substrates there was little digestion.
VII. Conformation Change of Cytochrome c during Oxidation and Reduc....................................... tion. A. Digestion of Yeast Cytochrome c by Bacterial Proteinme. . . B. Digestion of Reduced Yeast Cytochrome c by Bacterial Proteinase under Aerobic and Anaerobic Conditions. . . . . . . . C. Effect of TCA Treatment and Boiling on the Autoxidizability of Cytochrome c . ......................................... 29 30 32 32 32 35 35 37 37 38 39 39 41 43 44 44 44 46 48 50 58 62 62 64 30 KAXUO OKUNUKI CONTENTS (continued) D.
Advances in Enzymology and Related Areas of Molecular Biology, Volume 23 by Alton Meister