By Rodolfo Paoletti, Dr. David Kritchevsky
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Extra resources for Advances in lipid research. Volume 7
1 % rubeanic acid in 7 0 % ethanol for 30 minutes. Dissolve the rubeanic acid in ethanol, warm slightly (avoiding a naked flame), and make up to volume with distilled water. (e) Wash for a few minutes in 7 0 % ethanol and then wash in water. Mount in glycerine jelly, or dehydrate, clear and mount in DPX or balsam. Fatty acids are stained greenish-black. Method 6. , 1966) Triglycérides (a) Frozen sections are cut from tissue fixed in 1% calcium acetate-10% formalin or in "routine" formalin. (b) Incubate free-floating sections in the reaction medium for 2-4 hours at 37°C.
Cerebroside in myelin of callosal radiation, hippocampal radiation, and corpus striatum of rat brain. Section at left extracted with chloroform-methanol (2:1 v/v). Modified periodic acid-Schiff, X 6. ) Lipid Histochemistry 29 used as an identifying histochemical feature unless it can be shown that such basophilia is extinguished by extraction with lipid solvents. , 1966). The really distinctive chemical feature of sulfatide is its golden brown metachromasia when stained with an acetic acid-cresyl violet mixture (Peiffer and Hirsch, 1955; Hirsch and Peiffer, 1957).
FIG. 13. Sphingomyelin in rat cord myelin. NaOH-OTAN, phase-contrast, X 2360. ) Lipid Histochemistry 31 In summary, the NaOH-OTAN method stains sphingomyelin: preliminary mercuric chloride hydrolysis should exclude interference by plasmalogen and acetal phospholipids. 9. Lipofuscins It is not intended to discuss lipofuscins and ceroid in detail since the histochemistry of these lipid pigments has been extensively reviewed by Pearse (1960) and Wolman (1961, 1964). Ceroid and lipofuscin are probably formed by peroxidation of lipids in intracellular structures, which are then engulfed by lysosomes or phagolysosomes to appear as characteristic intracellular lipofuscin granules (see Adams, 1965c).
Advances in lipid research. Volume 7 by Rodolfo Paoletti, Dr. David Kritchevsky